To determine bacteriostatic abilities of Artemisia argyi extracts, and to explore the effect of Artemisia argyi extracts on oral ulcer in rats. Methods: We extracted the mixture of Artemisia argyi volatile oils and water-extraction by leaching method and evaluated the anti-microbial effect of Artemisia argyi extracts on common oral floras in vitro. The rat cheeks were burnt by NaOH to establish the models of oral ulcer. The curative effects of crude drug of Artemisia argyi extracts at 2.0, 1.0, 0.5 g/mL on oral ulcer in rats were evaluated by measuring the oral ulcer healing time. Serum TNF-α level and expression of proliferating cell nuclear antigen (PCNA) were analyzed by ELASA and immunohistochemical staining. Results: Artemisia argyi extracts obviously inhibited the Staphylococcus aureus and Streptococcus. NaOH-made oral ulcer in rats were successfully established. The crude drug at 2.0 and 1.0 g/mL obviously reduced healing time, significantly inhibited the release of TNF-α, and improved the PCNA level in the ulcer tissues (All P<0.01). The extracts obviously reduced the local inflammatory reaction and promoted tissue repair of oral ulcer. Conclusion: Artemisia argyi extracts promote tissue repair of oral ulcer via inhibiting bacterial growth, reducing the release of TNF-α and improving the PCNA level.
目的：体外研究艾叶提取物抑菌能力，初步探索艾叶提取物对大鼠口腔溃疡模型的治疗效果及其机制。方法：用浸出法提取艾叶挥发油及水提液混合物；体外研究艾叶提取物对口腔常见菌群的抑菌能力；以NaOH灼烧大鼠唇内侧造口腔溃疡模型，观察2.0，1.0，0.5 g/mL生药浓度的艾叶提取物对大鼠口腔溃疡模型愈合时间、溃疡修复程度的影响；运用ELASA法检测各组血清肿瘤坏死因子(tumor necrosis factor-α，TNF-α)含量，免疫组织化学法检测溃疡局部病变组织增殖细胞核抗原(proliferating cell nuclear antigen，PCNA)的表达情况。结果：艾叶提取物对金黄色葡萄球菌和链球菌生长具有显著的抑制作用；使用NaOH晶体成功构建大鼠口腔溃疡模型，2.0和1.0g/mL艾叶提取物均可有效缩短口腔溃疡愈合时间(P<0.01)，显著降低血清TNF-α表达(P<0.01)及提高溃疡局部PCNA表达水平(P<0.01)，有效减轻局部炎症反应并促进组织修复。结论：艾叶提取物能通过抑制细菌生长，降低血清TNF-α含量及提高溃疡局部PCNA表达来促进大鼠口腔溃疡的愈合。.