Asymmetric DNA methylation between sister chromatids of metaphase chromosomes in mouse embryos upon bisphenol A action

E L Patkin; N A Grudinina; L K Sasina; E M Noniashvili; L I Pavlinova; I O Suchkova; M E Kustova; N N Kolmakov; Tran Van Truong; G A Sofronov

doi:10.1016/j.reprotox.2017.08.017

Asymmetric DNA methylation between sister chromatids of metaphase chromosomes in mouse embryos upon bisphenol A action

Reprod Toxicol. 2017 Dec:74:1-9. doi: 10.1016/j.reprotox.2017.08.017. Epub 2017 Aug 24.

Authors

Affiliations

¹ Lab. of Molec. Cytogenet. of Mammal. Devel., Dep. of Molec. Genetics, Institute of Experimental Medicine of the Russian Academy of Sciences, akad. Pavlov str., 12, St. Petersburg, 197376, Russia. Electronic address: elp44@mail.ru.
² Lab. of Molec. Cytogenet. of Mammal. Devel., Dep. of Molec. Genetics, Institute of Experimental Medicine of the Russian Academy of Sciences, akad. Pavlov str., 12, St. Petersburg, 197376, Russia.
³ Lab. of Molec. Cytogenet. of Mammal. Devel., Dep. of Molec. Genetics, Institute of Experimental Medicine of the Russian Academy of Sciences, akad. Pavlov str., 12, St. Petersburg, 197376, Russia; Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, 199034, Russia.
⁴ Lab. of Molec. Cytogenet. of Mammal. Devel., Dep. of Molec. Genetics, Institute of Experimental Medicine of the Russian Academy of Sciences, akad. Pavlov str., 12, St. Petersburg, 197376, Russia; Vietnamese Tropical Research Centre, Viet Nam.

PMID: 28843703
DOI: 10.1016/j.reprotox.2017.08.017

Abstract

Earlier we showed that asymmetric methylation of sister chromatids (AMSC) was a specific characteristic of differentiation potency, and supposed that AMSC could be a useful marker of environmental impact connected with differentiation and/or dedifferentiation. Here we investigated the level of AMSC in chromosomes and the nuclei methylation in mouse preimplantation and postimplantation embryos, in comparison with the undifferentiated cells of mouse embryonal carcinoma cell line F9, and human differentiated HEK293 cells upon BPA influence. We found that exposure of mouse preimplantation embryos to BPA caused a significant decrease in the level of AMSC in chromosomes and the nuclei methylation. The BPA exposure of potentially differentiating F9 cells had no any influence on DNA methylation in nuclei but significantly decreased the number of AMSC. The level of DNA methylation and AMSC in HEK293 cells were not also changed. These data indicate that BPA exerts significant influence on differentiating and potentially differentiable cells. The most sensitive BPA targets are preimplantation embryos and stem cells.

Keywords: Asymmetric cell division; Asymmetric methylation of sister chromatids; Bisphenol A; DNA methylation; HEK293 cell line; Immunocytochemistry; Metaphase chromosomes; Methylcytosine; Mouse embryonic carcinoma cell line F9; Nuclei methylation; Preimplantation and postimplantation mouse embryo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzhydryl Compounds / toxicity*
Cell Line, Tumor
Chromatids / drug effects*
Chromatids / genetics
DNA Methylation / drug effects*
Embryo, Mammalian / drug effects*
Embryo, Mammalian / metabolism
Estrogens, Non-Steroidal / toxicity*
Female
HEK293 Cells
Humans
Metaphase
Mice
Phenols / toxicity*

Substances

Benzhydryl Compounds
Estrogens, Non-Steroidal
Phenols
bisphenol A