Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

Dokyoung Kim; Jinyoung Kang; Taejun Wang; Hye Gun Ryu; Jonathan M Zuidema; Jinmyoung Joo; Muwoong Kim; Youngbuhm Huh; Junyang Jung; Kyo Han Ahn; Ki Hean Kim; Michael J Sailor

doi:10.1002/adma.201703309

Two-Photon In Vivo Imaging with Porous Silicon Nanoparticles

Adv Mater. 2017 Oct;29(39). doi: 10.1002/adma.201703309. Epub 2017 Aug 21.

Authors

Dokyoung Kim^{1

2}, Jinyoung Kang³, Taejun Wang⁴, Hye Gun Ryu⁵, Jonathan M Zuidema⁶, Jinmyoung Joo^{7

8}, Muwoong Kim¹, Youngbuhm Huh¹, Junyang Jung¹, Kyo Han Ahn⁵, Ki Hean Kim⁹, Michael J Sailor¹⁰

Affiliations

¹ Department of Anatomy and Neurobiology, College of Medicine, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul, 02447, Republic of Korea.
² Center for Converging Humanities, Kyung Hee University, 26 Kyungheedae-Ro, Dongdaemun-Gu, Seoul, 02447, Republic of Korea.
³ Department of Nanoengineering, University of California, San Diego, La Jolla, CA, 92093-0358, USA.
⁴ Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Pohang, Gyungbuk, 37673, Republic of Korea.
⁵ Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk, 37673, Republic of Korea.
⁶ Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA, 92093-0358, USA.
⁷ Department of Convergence Medicine, University of Ulsan College of Medicine, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.
⁸ Biomedical Engineering Research Center, Asan Institute for Life Science, Asan Medical Center, 88 Olympic-ro 43-gil, Songpa-gu, Seoul, 05505, Republic of Korea.
⁹ Division of Integrative Biosciences and Biotechnology, Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Pohang, Gyungbuk, 37673, Republic of Korea.
¹⁰ Department of Chemistry and Biochemistry, Department of Nanoengineering, University of California, San Diego, La Jolla, CA, 92093-0358, USA.

PMID: 28833739
DOI: 10.1002/adma.201703309

Abstract

A major obstacle in luminescence imaging is the limited penetration of visible light into tissues and interference associated with light scattering and autofluorescence. Near-infrared (NIR) emitters that can also be excited with NIR radiation via two-photon processes can mitigate these factors somewhat because they operate at wavelengths of 650-1000 nm where tissues are more transparent, light scattering is less efficient, and endogenous fluorophores are less likely to absorb. This study presents photolytically stable, NIR photoluminescent, porous silicon nanoparticles with a relatively high two-photon-absorption cross-section and a large emission quantum yield. Their ability to be targeted to tumor tissues in vivo using the iRGD targeting peptide is demonstrated, and the distribution of the nanoparticles with high spatial resolution is visualized.

Keywords: cancer diagnostics; iRGD targeting peptide; nanomedicine; photoluminescence.