Biochar-induced negative carbon mineralization priming effects in a coastal wetland soil: Roles of soil aggregation and microbial modulation

Abstract

Biochar can sequestrate carbon (C) in soils and affect native soil organic carbon (SOC) mineralization via priming effects. However, the roles of soil aggregation and microbial regulation in priming effects of biochars on SOC in coastal wetland soils are poorly understood. Thus, a coastal wetland soil (δ¹³C -22‰) was separated into macro-micro aggregates (53-2000μm, MA) and silt-clay fractions (<53μm, SF) to investigate the priming effect using two ¹³C enriched biochars produced from corn straw (δ¹³C -11.58‰) at 350 and 550°C. The two biochars induced negative priming effect on the native SOC mineralization in the both soil aggregate size fractions, attributed to the enhanced stability of the soil aggregates resulting from the intimate physico-chemical associations between the soil minerals and biochar particles. Additionally, biochar amendments increased soil microbial biomass C and resulted in a lower metabolic quotient, suggesting that microbes in biochar amended aggregates could likely incorporate biomass C rather than mineralize it. Moreover, the biochar amendments induced obvious shifts of the bacterial community towards low C turnover bacteria taxa (e.g., Actinobacteria and Deltaproteobacteria) and the bacteria taxa responsible for stabilizing soil aggregates (e.g., Actinobacteria and Acidobacteria), which also accounted for the negative priming effect. Overall, these results suggested that biochar had considerable merit for stabilizing SOC in the coastal soil and thus has potential to restore and/or enhance "blue C" sink in the degraded coastal wetland ecosystem.

Keywords: Bacterial community; Blue carbon; Carbon sequestration; Remediation; Soil aggregate; Soil degradation.