Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles

Methods Mol Biol. 2017:1660:433-440. doi: 10.1007/978-1-4939-7253-1_35.

Abstract

Extracellular vesicles (EVs) are nanosized membrane vesicles, released by many cell types, which are an ideal drug-delivery system for antitumor therapy. Exosomes, microparticles (MP), and apoptotic bodies are the three main types of EVs. MPs are EVs with size ranges between 100 and 1000 nm. Tumor cell-derived EVs (T-EVs) can serve as a safe and efficient vehicle to specific deliver chemotherapeutic drugs or oncolytic adenoviruses for tumor treatment. In this section, we describe how to prepare EVs for therapeutic use.

Keywords: Chemotherapeutic drugs; Dendritic cells; Drug delivery; Extracellular vesicles; Oncolytic adenoviruses; Tumor antigens; Tumor microparticles; Tumor vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents / administration & dosage
  • Cancer Vaccines
  • Cell Line, Tumor
  • Cell-Derived Microparticles / metabolism*
  • Cell-Derived Microparticles / transplantation
  • Disease Models, Animal
  • Drug Delivery Systems
  • Extracellular Vesicles / metabolism*
  • Extracellular Vesicles / transplantation
  • Female
  • Gene Transfer Techniques
  • Humans
  • Mice
  • Neoplasms / metabolism*
  • Neoplasms / pathology
  • Neoplasms / therapy*
  • Oncolytic Virotherapy
  • Xenograft Model Antitumor Assays

Substances

  • Antineoplastic Agents
  • Cancer Vaccines