Small Prosthetic Groups in 18F-Radiochemistry: Useful Auxiliaries for the Design of 18F-PET Tracers

Abstract

Prosthetic group (PG) applications in ¹⁸F-radiochemistry play a pivotal role among current ¹⁸F^-labeling techniques for the development and availability of ¹⁸F-labeled imaging probes for PET (Wahl, 2002) (¹). The introduction and popularization of PGs in the mid-80s by pioneers in ¹⁸F-radiochemistry has profoundly changed the landscape of available tracers for PET and has led to a multitude of new imaging agents based on simple and efficiently synthesized PGs. Because of the chemical nature of anionic ¹⁸F^- (apart from electrophilic low specific activity ¹⁸F-fluorine), radiochemistry before the introduction of PGs was limited to simple nucleophilic substitutions of leaving group containing precursor molecules. These precursors were not always available, and some target compounds were either hard to synthesize or not obtainable at all. Even with the advent of recently introduced "late-stage fluorination" techniques for the ¹⁸F-fluorination of deactivated aromatic systems, PGs will continue to play a central role in ¹⁸F-radiochemistry because of their robust and almost universal usability. The importance of PGs in radiochemistry is shown by its current significance in tracer development and exemplified by an overview of selected methodologies for PG attachment to PET tracer molecules. Especially, click-chemistry approaches to PG conjugation, while furthering the historical evolution of PGs in PET tracer design, play a most influential role in modern PG utilization. All earlier and recent multifaceted approaches in PG development have significantly enriched the contingent of modern ¹⁸F-radiochemistry procedures and will continue to do so.