Glucose variability provides detailed information on glucose control and fluctuation. The aim of this study is to investigate the glucose variability by multi-scale analysis approach on 72-h glucose series captured by continuous glucose monitoring system (CGMS), gaining insights into the variability and complexity of the glucose time series data. Ninety-eight type 2 DM patients participated in this study, and 72-h glucose series from each subject were recorded by CGMS. Subjects were divided into two subgroups according to the mean amplitude of glycemic excursions (MAGE) value threshold at 3.9 based on Chinese standard. In this study, we applied two types of multiple scales analysis methods on glucose time series: ensemble empirical mode decomposition (EEMD) and refined composite multi-scale entropy (RCMSE). With EEMD, glucose series was decomposed into several intrinsic mode function (IMF), and glucose variability was examined on multiple time scales with periods ranging from 0.5 to 12 h. With RCMSE, complexity of the structure of glucose series was quantified at each time scale ranging from 5 to 30 min. Subgroup with higher MAGE value (>3.9) presented higher glycemic baseline and variability. There were significant differences in glycemic variability on IMFs3-5 between subgroups with MAGE>3.9 and MAGE < = 3.9 (p<0.001), but no significant differences in variability on IMFs1-2. The complexity of glucose series quantified by RCMSE showed statistically difference on each time scale from 5 to 30 min between subgroups (p<0.05). Glucose series from subjects with higher MAGE value represented higher variability but lower complexity on multiple time scales. Compared with traditional matrices measuring the glucose variability, approaches of EEMD and RCMSE can quantify the dynamic glycemic fluctuation in multiple time scales and provide us more detailed information on glycemic variability and complexity.
Keywords: Continuous glucose fluctuation; Ensemble empirical mode decomposition; Mean absolute glycemic excursions; Multiple scales; Refined composite multi-scale entropy.