Fusobacterium nucleatum detected simultaneously in a pyogenic liver abscess and advanced sigmoid colon cancer

Ryuta Shigefuku; Tsunamasa Watanabe; Yuki Kanno; Hiroki Ikeda; Hiroyasu Nakano; Nobuhiro Hattori; Kotaro Matsunaga; Nobuyuki Matsumoto; Shin-Ichi Kanno; Katsuhiko Nosho; Atsuko Hachiya; Yasumasa Iwatani; Tomoko Matsumori; Satoshi Tsukikawa; Ryoji Makizumi; Takehito Otsubo; Hiroyuki Yamamoto; Fumio Itoh

doi:10.1016/j.anaerobe.2017.08.010

Fusobacterium nucleatum detected simultaneously in a pyogenic liver abscess and advanced sigmoid colon cancer

Anaerobe. 2017 Dec:48:144-146. doi: 10.1016/j.anaerobe.2017.08.010. Epub 2017 Aug 16.

Authors

Ryuta Shigefuku¹, Tsunamasa Watanabe¹, Yuki Kanno¹, Hiroki Ikeda¹, Hiroyasu Nakano¹, Nobuhiro Hattori¹, Kotaro Matsunaga¹, Nobuyuki Matsumoto¹, Shin-Ichi Kanno², Katsuhiko Nosho², Atsuko Hachiya³, Yasumasa Iwatani³, Tomoko Matsumori⁴, Satoshi Tsukikawa⁴, Ryoji Makizumi⁴, Takehito Otsubo⁴, Hiroyuki Yamamoto⁵, Fumio Itoh¹

Affiliations

¹ Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan.
² Department of Gastroenterology, Sapporo Medical University School of Medicine, Japan.
³ Department of Infectious Diseases and Immunology, Clinical Research Center, National Hospital Organization Nagoya Medical Center, Japan.
⁴ Division of Gastroenterological and General Surgery, St. Marianna University School of Medicine, Kawasaki, Japan.
⁵ Division of Gastroenterology and Hepatology, Department of Internal Medicine, St. Marianna University School of Medicine, Kawasaki, Japan. Electronic address: h-yama@marianna-u.ac.jp.

PMID: 28823592
DOI: 10.1016/j.anaerobe.2017.08.010

Abstract

Fusobacterium nucleatum is an invasive, adherent, and pro-inflammatory anaerobic bacterium involved in various infections and colorectal cancer. We report a case with pyogenic liver abscess, diagnosed with advanced sigmoid colon cancer, in whom F. nucleatum was simultaneously detected. In this patient, F. nucleatum was systematically analyzed using the molecular biological techniques of metagenome analysis, conventional PCR, and microbial fluorescence in situ hybridization.

Keywords: Colon cancer; Fusobacterium nucleatum; Metagenome analysis; Microbial FISH; Pyogenic liver abscess.

Publication types

Case Reports

MeSH terms

Aged
Biopsy
Fusobacterium Infections / diagnosis*
Fusobacterium Infections / microbiology*
Fusobacterium nucleatum / classification
Fusobacterium nucleatum / genetics*
Humans
In Situ Hybridization, Fluorescence
Liver Abscess, Pyogenic / diagnosis*
Liver Abscess, Pyogenic / microbiology*
Male
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Sigmoid Neoplasms / microbiology*
Tomography, X-Ray Computed
Ultrasonography

Substances

RNA, Ribosomal, 16S