Abstract
The introduction of two alternative glycolate catabolic pathways in the chloroplasts of Arabidopsis thaliana rendered plants with increased biomass. To introduce these synthetic pathways, the selected genes were stepwise integrated in the nuclear genome of wild-type plants. These plants were transformed by Agrobacterium tumefaciens carrying the binary vectors using the floral dip method. Selection of transformants was conducted using different selection agents and the expression of the transgenes was confirmed by PCR and enzyme activity measurements.
Keywords:
Agrobacterium tumefaciens; Arabidopsis transformation; Binary vectors; PCR amplification.
MeSH terms
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Agrobacterium tumefaciens / genetics
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Agrobacterium tumefaciens / metabolism
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Alcohol Oxidoreductases / genetics
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Alcohol Oxidoreductases / metabolism
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Catalase / genetics
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Catalase / metabolism
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Chloroplasts / genetics*
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Chloroplasts / metabolism
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Cloning, Molecular / methods
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Gene Expression Regulation, Plant*
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Malate Synthase / genetics
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Malate Synthase / metabolism
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Metabolic Engineering / methods*
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Oxygen Consumption / genetics
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Photosynthesis / genetics
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Plant Leaves / genetics*
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Plant Leaves / metabolism
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Plants, Genetically Modified
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Plasmids / chemistry
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Plasmids / metabolism
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Promoter Regions, Genetic
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Ribulose-Bisphosphate Carboxylase / genetics*
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Ribulose-Bisphosphate Carboxylase / metabolism
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Transformation, Genetic
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Transgenes
Substances
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Recombinant Proteins
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Alcohol Oxidoreductases
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glycollate oxidase
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Catalase
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Malate Synthase
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Ribulose-Bisphosphate Carboxylase