Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling

Methods Mol Biol. 2017:1653:17-29. doi: 10.1007/978-1-4939-7225-8_2.

Abstract

To study photorespiration and to characterize related components, gene expression analysis is a central approach. An overview of the experimental setup, protocols, and methods we use to investigate photorespiration-associated gene expression is presented. Within this chapter, we describe simple procedures to experimentally alter the photorespiratory flux and provide protocols for transcriptomic analysis with a focus on genes encoding photorespiratory proteins as well as those induced by photorespiratory hydrogen peroxide (H2O2). Examples of typical results are presented and their significance to understanding redox signaling is discussed.

Keywords: CO2; Catalase; H2O2; Photorespiration; Redox signaling; Transcriptomics.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Carbon Dioxide / metabolism
  • Gene Expression Regulation, Plant*
  • Hydrogen Peroxide / pharmacology
  • Kinetics
  • Oligonucleotide Array Sequence Analysis / instrumentation
  • Oligonucleotide Array Sequence Analysis / methods*
  • Oxidation-Reduction
  • Oxygen Consumption / physiology*
  • Photosynthesis / physiology*
  • Plant Leaves / drug effects
  • Plant Leaves / genetics
  • Plant Leaves / growth & development
  • Plant Leaves / metabolism
  • Ribulose-Bisphosphate Carboxylase / genetics
  • Ribulose-Bisphosphate Carboxylase / metabolism
  • Seeds / drug effects
  • Seeds / genetics
  • Seeds / growth & development
  • Seeds / metabolism
  • Signal Transduction
  • Transcriptome*

Substances

  • Arabidopsis Proteins
  • Carbon Dioxide
  • Hydrogen Peroxide
  • Ribulose-Bisphosphate Carboxylase