In situ formation of injectable and porous heparin-based hydrogel

Meeichyn Goh; Younghee Kim; Kihak Gwon; Kiyoon Min; YoungMin Hwang; Giyoong Tae

doi:10.1016/j.carbpol.2017.06.126

In situ formation of injectable and porous heparin-based hydrogel

Carbohydr Polym. 2017 Oct 15:174:990-998. doi: 10.1016/j.carbpol.2017.06.126. Epub 2017 Jul 12.

Authors

Meeichyn Goh¹, Younghee Kim², Kihak Gwon³, Kiyoon Min⁴, YoungMin Hwang⁵, Giyoong Tae⁶

Affiliations

¹ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: chyn87fish@gist.ac.kr.
² School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: sonho0202@gmail.com.
³ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: khgwon@gist.ac.kr.
⁴ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: kymin324@gist.ac.kr.
⁵ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: ymhwang@gist.ac.kr.
⁶ School of Materials Science and Engineering, Gwangju Institute of Science and Technology, 123 Cheomdan-gwagiro, Buk-gu, Gwangju 61005, Republic of Korea. Electronic address: gytae@gist.ac.kr.

PMID: 28821157
DOI: 10.1016/j.carbpol.2017.06.126

Abstract

In this study, by combining photopolymerization and particle leaching technique, in situ formation of porous hydrogel with pore interconnectivity was demonstrated in vivo upon subcutaneous injection into the back of mice as well as in vitro. A precursor solution containing thiolated heparin, PEG-diacrylate (PEG-DA), and gelatin microparticles (GMPs) as a fast dissolving porogen were photopolymerized by visible-light-initiated thiol-ene reaction with eosin Y (EY) as a photo initiator and triethanolamine (TEOA) as a co-initiator. Formation of porous structure of the hydrogel after subsequent leaching of GMPs was confirmed in an animal model as well as in a physiological environment. The physical characteristics of the hydrogel were analyzed, and the acute in vivo biocompatibility of this system was characterized.

Keywords: Gelatin microparticles; Hydrogel; Injectable; Photopolymerization; Porous.