RareCyte® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semiautomated Image Analysis

Methods Mol Biol. 2017:1634:173-180. doi: 10.1007/978-1-4939-7144-2_14.

Abstract

The RareCyte CyteFinder instrument is an automated scanner that allows rapid identification of circulating tumor cells (CTCs) on microscope slides prepared by the AccuCyte process (see Chapter 13 ) and stained by immunofluorescence. Here, we present the workflow for CyteFinder scanning, analysis, and CyteMapper scan review which includes CTC confirmation and report generation.

Keywords: Circulating tumor cells; Fluorescence scanning; Image analysis; Scoring algorithm.

MeSH terms

  • Antibodies, Monoclonal / chemistry
  • Antibodies, Monoclonal / metabolism
  • Automation, Laboratory / instrumentation
  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / immunology
  • Biomarkers, Tumor / metabolism
  • Cell Count
  • Cell Line, Tumor
  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Cells, Immobilized / immunology
  • Cells, Immobilized / metabolism
  • Cells, Immobilized / pathology*
  • Centrifugation / instrumentation
  • Centrifugation / methods
  • Epithelial Cell Adhesion Molecule / genetics
  • Epithelial Cell Adhesion Molecule / immunology
  • Epithelial Cell Adhesion Molecule / metabolism
  • Equipment Design
  • Fluorescent Antibody Technique / methods
  • Fluorescent Dyes / chemistry
  • Humans
  • Image Processing, Computer-Assisted / methods*
  • Immunoconjugates / chemistry
  • Keratins / genetics
  • Keratins / immunology
  • Keratins / metabolism
  • Leukocyte Common Antigens / genetics
  • Leukocyte Common Antigens / immunology
  • Leukocyte Common Antigens / metabolism
  • Neoplasms / blood
  • Neoplasms / diagnosis*
  • Neoplasms / immunology
  • Neoplasms / pathology
  • Neoplastic Cells, Circulating / immunology
  • Neoplastic Cells, Circulating / metabolism
  • Neoplastic Cells, Circulating / pathology*
  • Protein Binding
  • Single-Cell Analysis / instrumentation
  • Single-Cell Analysis / methods*

Substances

  • Antibodies, Monoclonal
  • Biomarkers, Tumor
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Fluorescent Dyes
  • Immunoconjugates
  • Keratins
  • Leukocyte Common Antigens
  • PTPRC protein, human