Deep Sequencing Reveals a Divergent Ugandan cassava brown streak virus Isolate from Malawi

Willard Mbewe; Stephan Winter; Settumba Mukasa; Fred Tairo; Peter Sseruwagi; Joseph Ndunguru; Siobain Duffy

doi:10.1128/genomeA.00818-17

Deep Sequencing Reveals a Divergent Ugandan cassava brown streak virus Isolate from Malawi

Genome Announc. 2017 Aug 17;5(33):e00818-17. doi: 10.1128/genomeA.00818-17.

Authors

Willard Mbewe^{1

2}, Stephan Winter³, Settumba Mukasa², Fred Tairo⁴, Peter Sseruwagi⁴, Joseph Ndunguru⁴, Siobain Duffy⁵

Affiliations

¹ Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, New Jersey, USA mbewewillard@yahoo.co.uk.
² School of Agricultural Science, Makerere University, Kampala, Uganda.
³ DSMZ Plant Virus Department, Leibniz Institute, Braunschweig, Germany.
⁴ Mikocheni Agricultural Research Institute, Dar es Salaam, Tanzania.
⁵ Department of Ecology, Evolution and Natural Resources, Rutgers University, New Brunswick, New Jersey, USA.

Abstract

Illumina sequencing of RNA from a cassava cutting from northern Malawi produced a genome of Ugandan cassava brown streak virus (UCBSV-MW-NB7_2013). Sequence comparisons revealed stronger similarity to an isolate from nearby Tanzania (93.4% pairwise nucleotide identity) than to those previously reported from Malawi (86.9 to 87.0%).