Advances in Capillary Electrophoretically Mediated Microanalysis for On-line Enzymatic and Derivatization Reactions

Shengyun Huang; Prasanta Paul; Pranov Ramana; Erwin Adams; Patrick Augustijns; Ann Van Schepdael

doi:10.1002/elps.201700262

Advances in Capillary Electrophoretically Mediated Microanalysis for On-line Enzymatic and Derivatization Reactions

Electrophoresis. 2018 Jan;39(1):97-110. doi: 10.1002/elps.201700262. Epub 2017 Sep 13.

Authors

Shengyun Huang¹, Prasanta Paul¹, Pranov Ramana¹, Erwin Adams¹, Patrick Augustijns², Ann Van Schepdael¹

Affiliations

¹ KU Leuven - University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Pharmaceutical Analysis, Leuven, Belgium.
² KU Leuven - University of Leuven, Department of Pharmaceutical and Pharmacological Sciences, Drug delivery and disposition, Leuven, Belgium.

PMID: 28815641
DOI: 10.1002/elps.201700262

Abstract

This review summarizes recent developments, applications, and innovations of capillary electrophoretically mediated microanalysis methods. As a follow up of an earlier review, it covers the literature from early 2015 to early 2017. This article is divided into three parts. In the first part, different types of mixing procedures and applications of enzyme mediated microanalysis are discussed; the second part summarizes immobilized enzyme reactors (IMERs), while the third part deals with recent advances in on-line derivatization reactions.

Keywords: Capillary electrophoresis; Derivatization reactions; Electrophoretically mediated microanalysis; Immobilized enzyme reactors; On-line enzymatic analysis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Electrophoresis, Capillary / instrumentation
Electrophoresis, Capillary / methods*
Enzymes, Immobilized / chemistry*
High-Throughput Screening Assays / methods
Mass Spectrometry / methods
Microchemistry / methods
Surface Properties

Substances

Enzymes, Immobilized