Chicken Induced Pluripotent Stem Cells: Establishment and Characterization

Aurelie Fuet; Bertrand Pain

doi:10.1007/978-1-4939-7216-6_14

Chicken Induced Pluripotent Stem Cells: Establishment and Characterization

Methods Mol Biol. 2017:1650:211-228. doi: 10.1007/978-1-4939-7216-6_14.

Authors

Aurelie Fuet¹, Bertrand Pain²

Affiliations

¹ Univ Lyon, Université Lyon 1, INSERM, INRA, Stem Cell and Brain Research Institute, U1208, USC1361, 69500, Bron, France.
² Univ Lyon, Université Lyon 1, INSERM, INRA, Stem Cell and Brain Research Institute, U1208, USC1361, 69500, Bron, France. bertrand.pain@inserm.fr.

PMID: 28809024
DOI: 10.1007/978-1-4939-7216-6_14

Abstract

In mammals, the introduction of the OSKM (Oct4, Sox2, Klf4, and c-Myc) genes into somatic cells has allowed generating induced pluripotent stem (iPS) cells. So far, this process has been only clearly demonstrated in mammals. Here, using chicken as an avian model, we describe a set of protocols allowing the establishment, characterization, maintenance, differentiation, and injection of putative reprogrammed chicken Induced Pluripotent Stem (iPS) cells.

Keywords: Chicken; Induced pluripotent stem cells; Transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation*
Cells, Cultured
Cellular Reprogramming*
Chickens
Gene Expression Regulation*
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism*
Signal Transduction