Best practices in cell culture: an overview

In Vitro Cell Dev Biol Anim. 2017 Sep;53(8):669-672. doi: 10.1007/s11626-017-0177-7. Epub 2017 Aug 14.

Abstract

This overview describes a series of articles to provide an unmet need for information on best practices in animal cell culture. The target audience primarily consists of entry-level scientists with minimal experience in cell culture. It also include scientists, journalists, and educators with some experience in cell culture, but in need of a refresher in best practices. The articles will be published in this journal over a six-month period and will emphasize best practices in: (1) media selection; (2) use and evaluation of animal serum as a component of cell culture medium; (3) receipt of new cells into the laboratory; (4) naming cell lines; (5) authenticating cell line identity; (6) detecting and mitigating risk of cell culture contamination; (7) cryopreservation and thawing of cells; and (8) storing and shipping viable cells.

Keywords: Best practices in cell culture; Overview.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Culture Techniques / trends
  • Cell Line
  • Cryopreservation / methods*
  • Culture Media*

Substances

  • Culture Media