Mycoplasma hyopneumoniae detection in nylon-flocked and rayon-bud swabs

Karine L Takeuti; David E S N de Barcellos; Maria Pieters

doi:10.1016/j.mimet.2017.08.007

Mycoplasma hyopneumoniae detection in nylon-flocked and rayon-bud swabs

J Microbiol Methods. 2017 Oct:141:118-120. doi: 10.1016/j.mimet.2017.08.007. Epub 2017 Aug 12.

Authors

Karine L Takeuti¹, David E S N de Barcellos², Maria Pieters³

Affiliations

¹ Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States.
² Department of Animal Medicine, College of Veterinary Medicine, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil.
³ Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, United States. Electronic address: piet0094@umn.edu.

PMID: 28807761
DOI: 10.1016/j.mimet.2017.08.007

Abstract

The objective of this study was to compare the absorption and M. hyopneumoniae detection by real-time PCR using nylon-flocked and rayon-bud swabs. Results of this study showed that absorption and detection of M. hyopneumoniae were significantly higher in nylon-flocked swabs with Ct differences only ranging from 0.5 to 1.7.

Keywords: Diagnostics; Enzootic pneumonia; Real-time PCR; Swabs; Swine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques*
Cellulose*
Mycoplasma hyopneumoniae / genetics
Mycoplasma hyopneumoniae / isolation & purification*
Mycoplasma hyopneumoniae / physiology
Nylons*
Pneumonia of Swine, Mycoplasmal / diagnosis*
Pneumonia of Swine, Mycoplasmal / microbiology
Real-Time Polymerase Chain Reaction
Swine

Substances

Nylons
Cellulose
rayon, purified