A simple methodology to collect culturable bacteria from feces of Anopheles darlingi (Diptera: Culicidae)

Andrelisse Arruda; Gabriel Sousa Ferreira; Nucia Cristiane da Silva Lima; Antonio Dos Santos Júnior; Márlon Grégori Flores Custódio; Najla Benevides-Matos; Luiz Shozo Ozaki; Rodrigo G Stabeli; Alexandre A Silva

doi:10.1016/j.mimet.2017.08.004

A simple methodology to collect culturable bacteria from feces of Anopheles darlingi (Diptera: Culicidae)

J Microbiol Methods. 2017 Oct:141:115-117. doi: 10.1016/j.mimet.2017.08.004. Epub 2017 Aug 12.

Authors

Andrelisse Arruda¹, Gabriel Sousa Ferreira², Nucia Cristiane da Silva Lima³, Antonio Dos Santos Júnior⁴, Márlon Grégori Flores Custódio³, Najla Benevides-Matos³, Luiz Shozo Ozaki⁵, Rodrigo G Stabeli³, Alexandre A Silva²

Affiliations

¹ Universidade Federal de Rondônia, Porto Velho, RO, Brazil; Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brazil. Electronic address: andrelisse.arruda@gmail.com.
² Universidade Federal de Rondônia, Porto Velho, RO, Brazil.
³ Fundação Oswaldo Cruz, Fiocruz Rondônia, Porto Velho, RO, Brazil.
⁴ Instituto Federal de Rondônia-IFRO/Campus Porto Velho Calama, Porto Velho, RO, Brazil.
⁵ Virginia Commonwealth University, Richmond, VA, USA.

PMID: 28807760
DOI: 10.1016/j.mimet.2017.08.004

Abstract

A simple methodology based in a modified mosquito cage with a Petri dish containing culture medium was successfully used as an alternative method to the traditional digestive tract dissection protocol to collect bacteria from the feces of the mosquito Anopheles darlingi.

Keywords: Anopheles; Bacteria; Microbiota; Mosquito; Paratransgenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anopheles / microbiology*
Bacteria / growth & development*
Bacteria / isolation & purification*
Bacteriological Techniques*
Culture Media
Feces / microbiology*
Gastrointestinal Microbiome

Substances

Culture Media