Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique

Curr Protoc Stem Cell Biol. 2017 Aug 14:42:4A.14.1-4A.14.7. doi: 10.1002/cpsc.32.

Abstract

This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. © 2017 by John Wiley & Sons, Inc.

Keywords: Matrigel; cardiac; hiPSC-CMs; myocytes; stem cells.

MeSH terms

  • Cell Culture Techniques / methods*
  • Collagen / chemistry*
  • Drug Combinations
  • Humans
  • Induced Pluripotent Stem Cells / cytology
  • Induced Pluripotent Stem Cells / metabolism*
  • Laminin / chemistry*
  • Myocardial Contraction*
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / metabolism*
  • Proteoglycans / chemistry*

Substances

  • Drug Combinations
  • Laminin
  • Proteoglycans
  • matrigel
  • Collagen