Fed-batch production of vanillin by Bacillus aryabhattai BA03

Alicia Paz; David Outeiriño; Ricardo Pinheiro de Souza Oliveira; José Manuel Domínguez

doi:10.1016/j.nbt.2017.07.012

Fed-batch production of vanillin by Bacillus aryabhattai BA03

N Biotechnol. 2018 Jan 25;40(Pt B):186-191. doi: 10.1016/j.nbt.2017.07.012. Epub 2017 Aug 10.

Authors

Alicia Paz¹, David Outeiriño¹, Ricardo Pinheiro de Souza Oliveira², José Manuel Domínguez³

Affiliations

¹ Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain; Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain.
² Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, Bl 16, 05508-900 São Paulo, Brazil.
³ Department of Chemical Engineering, Faculty of Sciences, University of Vigo (Campus Ourense), As Lagoas s/n, 32004 Ourense, Spain; Laboratory of Agro-food Biotechnology, CITI (University of Vigo)-Tecnópole, Parque Tecnológico de Galicia, San Cibrao das Viñas, 32900 Ourense, Spain. Electronic address: jmanuel@uvigo.es.

PMID: 28803126
DOI: 10.1016/j.nbt.2017.07.012

Abstract

Bacillus aryabhattai BA03, a strain isolated in our laboratory, has interesting properties related to the production of natural aromas and flavors. Specifically, we have found that it was able to produce vanillin from ferulic acid (FA). Furthermore, this strain produces high amounts of 4-vinylguaiacol in only 14h, this being the only intermediate metabolite observed in the process. FA is an inexpensive feedstock for the production of natural value-added compounds when extracted from lignocellulosic wastes. In this study, we optimized the operational conditions (temperature, pH and agitation), medium composition and bioconversion technology (batch or fed-batch) to produce vanillin. In a fed-batch process conducted with just one additional supplementation after 24h, the maximal concentration of vanillin (147.1±0.9mg/L) was observed after 216h (Q_V=0.681mg/Lh; Y_V/fFA=0.082mg/mg) after degrading 90.3% FA. In view of our data, we postulate that Bacillus aryabhattai BA03 carries out a decarboxylation of ferulic acid as a metabolic pathway.

Keywords: 4-Vynilguaiacol; Bacillus aryabhattai; Decarboxylation; Ferulic acid; Vanillin.

MeSH terms

Bacillus / chemistry
Bacillus / metabolism*
Benzaldehydes / chemistry
Benzaldehydes / metabolism*
Coumaric Acids / chemistry
Coumaric Acids / metabolism
Hydrogen-Ion Concentration
Temperature

Substances

Benzaldehydes
Coumaric Acids
ferulic acid
vanillin