Upgrading of the symbiosis of Nitrosomanas and anammox bacteria in a novel single-stage partial nitritation-anammox system: Nitrogen removal potential and Microbial characterization

Yuan Liu; Qigui Niu; Shaopo Wang; Jiayuan Ji; Yu Zhang; Min Yang; Toshimasa Hojo; Yu-You Li

doi:10.1016/j.biortech.2017.07.156

Upgrading of the symbiosis of Nitrosomanas and anammox bacteria in a novel single-stage partial nitritation-anammox system: Nitrogen removal potential and Microbial characterization

Bioresour Technol. 2017 Nov;244(Pt 1):463-472. doi: 10.1016/j.biortech.2017.07.156. Epub 2017 Jul 29.

Authors

Yuan Liu¹, Qigui Niu², Shaopo Wang³, Jiayuan Ji⁴, Yu Zhang⁵, Min Yang⁵, Toshimasa Hojo⁴, Yu-You Li⁶

Affiliations

¹ State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Post Office Box 2871, Beijing 100085, China; Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Sendai 980-8579, Japan; Tianjin Key Laboratory of Aquatic Science and Technology, 26# Jinjing Road, Tianjin 300384, China.
² School of Environmental Science and Engineering, Shandong University, 27# Shanda South Road, Jinan 250100, China.
³ Tianjin Key Laboratory of Aquatic Science and Technology, 26# Jinjing Road, Tianjin 300384, China; Tianjin Key laboratory of Aquatic Science and Technology, School of Environmental and Municipal Engineering, Tianjin Chengjian University, 26# Jinjing Road, Tianjin 300384, China.
⁴ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Sendai 980-8579, Japan.
⁵ State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Post Office Box 2871, Beijing 100085, China.
⁶ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Sendai 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 28803096
DOI: 10.1016/j.biortech.2017.07.156

Abstract

A novel single-stage partial nitritation-anammox process equipped with porous functional suspended carriers was developed at 25°C in a CSTR by controlling dissolved oxygen <0.3mg/L. The nitrogen removal performance was almost unchanged over a nitrogen loading rate ranging from 0.5 to 2.5kgNH₄⁺-N/m³/d with a high nitrogen removal efficiency of 81.1%. The specific activity of AOB and anammox bacteria was of 3.00g-N/g-MLVSS/d (the suspended sludge), 3.56g-N/g-MLVSS/d (the biofilm sludge), respectively. The results of pyrosequencing revealed that Nitrosomonas (5.66%) and Candidatus_Kuenenia (4.95%) were symbiotic in carriers while Nitrosomonas (40.70%) was predominant in the suspended flocs. Besides, two specific types of heterotrophic filamentous bacteria in the suspended flocs (Haliscomenobacter) and the functional carrier biofilm (Longilinea) were shown to confer structural integrity to the aggregates. The novel single-stage partial nitritation-anammox process equipped with functional suspended carriers was shown to have good potential for the nitrogen-rich wastewater treatment.

Keywords: Biofilm carriers; Candidatus_Kuenenia; Nitrosomanas; Single-stage partial nitritation–anammox; Symbiosis.

MeSH terms

Bacteria
Bioreactors*
Denitrification*
Nitrogen
Symbiosis*

Substances

Nitrogen