Antimicrobial susceptibility of Clostridium difficile isolates from ICU colonized patients revealed alert to ST-37 (RT 017) isolates

Jingyu Yang; Xiaoxia Zhang; Xiaohua Liu; Lin Cai; Ping Feng; Xiaohui Wang; Zhiyong Zong

doi:10.1016/j.diagmicrobio.2017.06.021

Antimicrobial susceptibility of Clostridium difficile isolates from ICU colonized patients revealed alert to ST-37 (RT 017) isolates

Diagn Microbiol Infect Dis. 2017 Oct;89(2):161-163. doi: 10.1016/j.diagmicrobio.2017.06.021. Epub 2017 Jul 1.

Authors

Jingyu Yang¹, Xiaoxia Zhang², Xiaohua Liu³, Lin Cai⁴, Ping Feng⁵, Xiaohui Wang⁶, Zhiyong Zong⁷

Affiliations

¹ Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.
² Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.
³ Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Department of Clinical Microbiology, Xindu District Hospital, Chengdu, China.
⁴ Intensive Care Unit, West China Hospital, Sichuan University, Chengdu, China.
⁵ Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
⁶ Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China. Electronic address: wang_xiaohui@scu.edu.cn.
⁷ Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China; Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China; Department of Infection Control, West China Hospital, Sichuan University, Chengdu, China.

PMID: 28800896
DOI: 10.1016/j.diagmicrobio.2017.06.021

Abstract

Seventy Clostridium difficile isolates from ICU colonized patients were tested for antimicrobial susceptibility and screened for resistance determinants. We found that multilocus sequence type 37 (ribotype 017) toxin A-negative/B-positive isolates were more likely resistant to moxifloxacin than toxin A-positive/B-positive isolates (41.7% versus 9.3%) with major variations in both GyrA (Thr82Ile) and GyrB (Ser366Ala), suggesting that the use of quinolone should be more strictly regulated.

Keywords: Antimicrobial susceptibility; Clostridium difficile; ICU; Resistant determinants.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics*
Bacterial Toxins / genetics*
Clostridioides difficile / drug effects*
Clostridioides difficile / genetics
Clostridioides difficile / isolation & purification
Clostridium Infections / drug therapy
Clostridium Infections / microbiology
DNA Gyrase / genetics*
Drug Resistance, Multiple, Bacterial / genetics*
Enterotoxins / genetics*
Fluoroquinolones / pharmacology
Humans
Intensive Care Units
Microbial Sensitivity Tests
Moxifloxacin
Multilocus Sequence Typing
Quinolones / therapeutic use

Substances

Anti-Bacterial Agents
Bacterial Proteins
Bacterial Toxins
Enterotoxins
Fluoroquinolones
Quinolones
tcdA protein, Clostridium difficile
toxB protein, Clostridium difficile
DNA Gyrase
Moxifloxacin