Quick, sensitive serial NMR experiments with Radon transform

Rupashree Dass; Paweł Kasprzak; Krzysztof Kazimierczuk

doi:10.1016/j.jmr.2017.07.011

Quick, sensitive serial NMR experiments with Radon transform

J Magn Reson. 2017 Sep:282:114-118. doi: 10.1016/j.jmr.2017.07.011. Epub 2017 Jul 31.

Authors

Rupashree Dass¹, Paweł Kasprzak², Krzysztof Kazimierczuk³

Affiliations

¹ Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland.
² Department of Mathematical Methods in Physics, Faculty of Physics, University of Warsaw, Pasteura 5, Warsaw, Poland.
³ Centre of New Technologies, University of Warsaw, Banacha 2C, 02-097 Warsaw, Poland. Electronic address: k.kazimierczuk@cent.uw.edu.pl.

PMID: 28797925
DOI: 10.1016/j.jmr.2017.07.011

Abstract

The Radon transform is a potentially powerful tool for processing the data from serial spectroscopic experiments. It makes it possible to decode the rate at which frequencies of spectral peaks shift under the effect of changing conditions, such as temperature, pH, or solvent. In this paper we show how it also improves speed and sensitivity, especially in multidimensional experiments. This is particularly important in the case of low-sensitivity techniques, such as NMR spectroscopy. As an example, we demonstrate how Radon transform processing allows serial measurements of ¹⁵N-HSQC spectra of unlabelled peptides that would otherwise be infeasible.

Keywords: HSQC; Multidimensional spectroscopy; Radon transform; Variable temperature.

Publication types

Research Support, Non-U.S. Gov't