Photochemical Acceleration of DNA Strand Displacement by Using Ultrafast DNA Photo-crosslinking

Shigetaka Nakamura; Hirokazu Hashimoto; Satoshi Kobayashi; Kenzo Fujimoto

doi:10.1002/cbic.201700430

Photochemical Acceleration of DNA Strand Displacement by Using Ultrafast DNA Photo-crosslinking

Chembiochem. 2017 Oct 18;18(20):1984-1989. doi: 10.1002/cbic.201700430. Epub 2017 Aug 29.

Authors

Shigetaka Nakamura¹, Hirokazu Hashimoto¹, Satoshi Kobayashi², Kenzo Fujimoto¹

Affiliations

¹ School of Materials Science, Japan Advanced Institute Science and Technology, 1-1, Asahidai, Nomi, Ishikawa, 923-1292, Japan.
² Department of Computer Science, University of Electro-Communications, 1-1-1, Chofugaoka, Chofu, Tokyo, 182-8585, Japan.

PMID: 28796345
DOI: 10.1002/cbic.201700430

Abstract

DNA strand displacement is an essential reaction in genetic recombination, biological processes, and DNA nanotechnology. In particular, various DNA nanodevices enable complicated calculations. However, it takes time before the output is obtained, so acceleration of DNA strand displacement is required for a rapid-response DNA nanodevice. Herein, DNA strand displacement by using DNA photo-crosslinking to accelerate this displacement is evaluated. The DNA photo-crosslinking of 3-cyanovinylcarbazole (^CNV K) was accelerated at least 20 times, showing a faster DNA strand displacement. The rate of photo-crosslinking is a key factor and the rate of DNA strand displacement is accelerated through ultrafast photo-crosslinking. The rate of DNA strand displacement was regulated by photoirradiation energy.

Keywords: DNA; nucleic acids; photochemistry; strand displacement; ultrafast photo-crosslinking.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Cross-Linking Reagents / pharmacology*
DNA / chemistry*
DNA / genetics
Kinetics
Photochemical Processes*

Substances

Cross-Linking Reagents
DNA