Abstract
Aim:
We report the first outbreak caused by Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae sequence type 258 (ST 258) clone that occurred in Croatia from May to December 2012.
Materials & methods:
23 carbapenem-resistant K. pneumoniae isolates were recovered from clinical and screening specimens of 12 patients hospitalized in a regional hospital. The first isolates from the 12 patients were typed using pulsed-field gel electrophoresis and multilocus sequence typing.
Results:
PCR detection showed that all isolates harbored the bla KPC-2 gene, together with bla TEM-116 and bla SHV-11.
Conclusion:
The rapid detection of KPC-producing K. pneumoniae and vigorous implementation of infection control measures were necessary to successfully control the outbreak.
Keywords:
KPC-producing Klebsiella pneumoniae; drug-resistant bacterial infections; outbreak.
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Bacterial Typing Techniques
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Carbapenems / pharmacology
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Croatia / epidemiology
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DNA, Bacterial
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Disease Outbreaks*
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Drug Resistance, Bacterial
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Electrophoresis, Gel, Pulsed-Field
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Genes, Bacterial / genetics
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Hospitals
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Humans
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Klebsiella Infections / diagnosis
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Klebsiella Infections / epidemiology*
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Klebsiella pneumoniae / enzymology*
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Klebsiella pneumoniae / genetics*
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Klebsiella pneumoniae / isolation & purification
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Klebsiella pneumoniae / pathogenicity
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Microbial Sensitivity Tests
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Molecular Epidemiology
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Multilocus Sequence Typing
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Polymerase Chain Reaction
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beta-Lactam Resistance / genetics
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beta-Lactamases / genetics*
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beta-Lactamases / isolation & purification*
Substances
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Bacterial Proteins
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Carbapenems
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DNA, Bacterial
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beta-lactamase SHV-11
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beta-Lactamases
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beta-lactamase TEM-116, Pseudomonas aeruginosa
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carbapenemase
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carbapenemase-2, Klebsiella pneumoniae