Transcriptome of melanoma cells from two mouse models, Tyr:NRasQ61K and Tyr:Rack1-HA, Tyr:NRasQ61K

Cécil Campagne; Stéphanie Pons; Diane Esquerre; Jordi Estellé; Emmanuelle Bourneuf; Uwe Maskos; Giorgia Egidy

doi:10.1016/j.dib.2017.07.006

Transcriptome of melanoma cells from two mouse models, Tyr:NRas^Q61K and Tyr:Rack1-HA, Tyr:NRas^Q61K

Data Brief. 2017 Jul 11:14:32-34. doi: 10.1016/j.dib.2017.07.006. eCollection 2017 Oct.

Authors

Cécil Campagne^{1

2}, Stéphanie Pons³, Diane Esquerre⁴, Jordi Estellé⁵, Emmanuelle Bourneuf^{5

6}, Uwe Maskos³, Giorgia Egidy^{1

2

5}

Affiliations

¹ INRA, UMR955 Génétique Fonctionnelle et Médicale, Ecole Nationale Vétérinaire d'Alfort, F-94704 Maisons-Alfort, France.
² Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, UMR955 Génétique Fonctionnelle et Médicale, F-94704 Maisons-Alfort, France.
³ Unité Neurobiologie Intégrative des Systèmes Cholinergiques, URA2182, CNRS, Institut Pasteur, F75724 Paris Cedex 15, France.
⁴ GenPhySE, Université de Toulouse, INRA, INPT, ENVT, Castanet Tolosan, France.
⁵ GABI, INRA, AgroParisTech, Université Paris-Saclay, F-78352 Jouy-en-Josas, France.
⁶ LREG, CEA, Université Paris-Saclay, F-78352 Jouy-en-Josas, France.

Abstract

The transcriptome sequencing of melanoma cells from two mouse models differing in the expression level of the scaffold protein Receptor for activated C kinase (RACK1) are presented. Primary melanoma cells were harvested from Tyr:NRas^Q61K; Pax3^GFP/+ mice, with or without the Tyr:Rack1-HA transgene. Cells were cultured and infected with scramble shRNA or Rack1-targeting shRNA, on technical triplicates of viral infection. Libraries were prepared by selecting polyadenylated mRNAs and RNA Sequencing (RNASeq) was performed. Samples are described in the SRA portal (SRP096162) and FASTQ files have been deposited in Sequence Read Archive (accession numbers: SRR5150106 to SRR5150117). The interpretation of these data is presented in the following research article: "RACK1 cooperates with NRAS^Q61K to promote melanoma in vivo" (Campagne et al., 2017, doi: 10.1016/j.cellsig.2017.03.015) [1].

Keywords: Cutaneous melanoma; RACK1; RNAseq; shRNA.