Derivatisation for separation and detection in capillary electrophoresis (2015-2017)

Alain Wuethrich; Joselito P Quirino

doi:10.1002/elps.201700252

Derivatisation for separation and detection in capillary electrophoresis (2015-2017)

Electrophoresis. 2018 Jan;39(1):82-96. doi: 10.1002/elps.201700252. Epub 2017 Aug 25.

Authors

Alain Wuethrich¹, Joselito P Quirino²

Affiliations

¹ Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), University of Queensland, Brisbane, QLD, Australia.
² Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Hobart, TAS, Australia.

PMID: 28758685
DOI: 10.1002/elps.201700252

Abstract

Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

Keywords: Analyte modification; Capillary electrophoresis; Derivatisation; Sensitivity enhancement; Sequential injection; Stacking.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Electrophoresis, Capillary
Indicators and Reagents / chemistry
Isomerism
Mass Spectrometry / methods
Organic Chemicals / chemistry*
Organic Chemicals / isolation & purification*
Spectrometry, Fluorescence / methods
Spectrophotometry, Ultraviolet / methods
Surface Properties

Substances

Indicators and Reagents
Organic Chemicals