Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.)

Minarni; I Made Artika; Heddy Julistiono; Nurliani Bermawie; Eny Ida Riyanti; Hasim; Akhmad Endang Zainal Hasan

doi:10.1016/j.apjtm.2017.06.004

Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.)

Asian Pac J Trop Med. 2017 Jun;10(6):566-571. doi: 10.1016/j.apjtm.2017.06.004. Epub 2017 Jun 20.

Authors

Minarni¹, I Made Artika², Heddy Julistiono³, Nurliani Bermawie⁴, Eny Ida Riyanti⁵, Hasim¹, Akhmad Endang Zainal Hasan¹

Affiliations

¹ Department of Biochemistry, Faculty of Mathematics and Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia.
² Department of Biochemistry, Faculty of Mathematics and Sciences, Bogor Agricultural University, Darmaga Campus, Bogor 16680, Indonesia. Electronic address: imart@ipb.ac.id.
³ Research Center for Biology, The Indonesian Institute of Sciences (LIPI), Cibinong, Bogor, Indonesia.
⁴ Research Institute of Medicinal and Aromatic Plants, Agricultural Research Agency, Bogor, Indonesia.
⁵ Center for Biotechnology and Genetic Resources of Agriculture, Agricultural Research Agency, Bogor, Indonesia.

PMID: 28756920
DOI: 10.1016/j.apjtm.2017.06.004

Abstract

Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf (Annona muricata L.).

Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7 (Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. At an extract concentration of 100 μg/mL, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further IC₅₀ determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/mL, 50 μg/mL, 100 μg/mL, 200 μg/mL and 400 μg/mL.

Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an IC₅₀ of 19.20 μg/mL. The best isolates were screened again using a normal cell (Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp.

Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.

Keywords: Annona muricata L.; Anticancer MCF-7; Endophytic fungi; Soursop leaf.