A photoclickable peptide microarray platform for facile and rapid screening of 3-D tissue microenvironments

Sadhana Sharma; Michael Floren; Yonghui Ding; Kurt R Stenmark; Wei Tan; Stephanie J Bryant

doi:10.1016/j.biomaterials.2017.07.025

A photoclickable peptide microarray platform for facile and rapid screening of 3-D tissue microenvironments

Biomaterials. 2017 Oct:143:17-28. doi: 10.1016/j.biomaterials.2017.07.025. Epub 2017 Jul 20.

Authors

Sadhana Sharma¹, Michael Floren², Yonghui Ding³, Kurt R Stenmark⁴, Wei Tan⁵, Stephanie J Bryant⁶

Affiliations

¹ Department of Chemical and Biological Engineering, University of Colorado-Boulder, Boulder, CO, 80309, USA.
² Department of Mechanical Engineering, University of Colorado-Boulder, Boulder, CO, 80309, USA; Cardiovascular Pulmonary Research Laboratories, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA.
³ Department of Mechanical Engineering, University of Colorado-Boulder, Boulder, CO, 80309, USA.
⁴ Cardiovascular Pulmonary Research Laboratories, University of Colorado Anschutz Medical Campus, Aurora, CO, 80045, USA; Departments of Pediatrics and Medicine, University of Colorado, Anschutz Medical Campus, Aurorad, CO, 80045, USA.
⁵ Department of Mechanical Engineering, University of Colorado-Boulder, Boulder, CO, 80309, USA. Electronic address: wtan@colorado.edu.
⁶ Department of Chemical and Biological Engineering, University of Colorado-Boulder, Boulder, CO, 80309, USA; Material Science and Engineering Program, University of Colorado-Boulder, Boulder, CO, 80309, USA; BioFrontiers Institute, University of Colorado-Boulder, Boulder, CO, 80309, USA. Electronic address: stephanie.bryant@colorado.edu.

PMID: 28756193
DOI: 10.1016/j.biomaterials.2017.07.025

Abstract

Microarrays are powerful experimental tools for high-throughput screening of cellular behavior in multivariate microenvironments. Here, we present a new, facile and rapid screening method for probing cellular behavior in 3D tissue microenvironments. This method utilizes a photoclickable peptide microarray platform developed using electrospun fibrous poly(ethylene glycol) hydrogels and microarray contact printing. We investigated the utility of this platform with five different peptide motifs and ten cell types including stem, terminally differentiated, cancer or immune cells that were from either primary origin or cell lines and from different species. We validated the capabilities of this platform to screen arrays consisting of multiple peptide motifs and concentrations for selectivity to cellular adhesion and morphology. Moreover, this platform is amenable to controlled spatial presentation of peptides. We show that by leveraging the differential attachment affinities for two cell types to two different peptides, this platform can also be used to investigate cell-cell interactions through miniature co-culture peptide arrays. Our fibrous peptide microarray platform enables high-throughput screening of 3D tissue microenvironments in a facile and rapid manner to investigate cell-matrix interactions and cell-cell signaling and to identify optimal tissue microenvironments for cell-based therapies.

Keywords: Cell-cell interaction; Cell-matrix interaction; Fibrous hydrogel; High-throughput screening; Peptide microarray; Photoclickable; Tissue microenvironments.

MeSH terms

Animals
Cattle
Cell Adhesion
Cell Communication
Cell Culture Techniques / instrumentation*
Cell Culture Techniques / methods
Cell Line
Cell Line, Tumor
Cells, Cultured
Coculture Techniques / instrumentation
Coculture Techniques / methods
Equipment Design
High-Throughput Screening Assays / instrumentation*
High-Throughput Screening Assays / methods
Humans
Hydrogels / chemistry*
Mice
Optical Imaging / instrumentation*
Optical Imaging / methods
Peptides / chemistry*
Photochemical Processes
Polyethylene Glycols / chemistry*
Protein Array Analysis / instrumentation*
Protein Array Analysis / methods
Tissue Scaffolds / chemistry

Substances

Hydrogels
Peptides
Polyethylene Glycols