Microfluidic-Mass Spectrometry Interfaces for Translational Proteomics

R Daniel Pedde; Huiyan Li; Christoph H Borchers; Mohsen Akbari

doi:10.1016/j.tibtech.2017.06.006

Microfluidic-Mass Spectrometry Interfaces for Translational Proteomics

Trends Biotechnol. 2017 Oct;35(10):954-970. doi: 10.1016/j.tibtech.2017.06.006. Epub 2017 Jul 26.

Authors

R Daniel Pedde¹, Huiyan Li², Christoph H Borchers³, Mohsen Akbari⁴

Affiliations

¹ Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada; University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, 3101-4464 Markham St., Victoria, BC, V8Z 7X8, Canada.
² University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, 3101-4464 Markham St., Victoria, BC, V8Z 7X8, Canada.
³ University of Victoria-Genome British Columbia Proteomics Centre, University of Victoria, 3101-4464 Markham St., Victoria, BC, V8Z 7X8, Canada; Department of Biochemistry and Microbiology, University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada; Gerald Bronfman Department of Oncology, McGill University, 5100 de Maisonneuve Blvd. West, Suite 720, Montreal, QC, H4A 3T2, Canada; Proteomics Centre, Jewish General Hospital, McGill University, 3755 Cote-Ste-Catherine Road, Montreal, QC, H3T 1E2, Canada. Electronic address: christoph@proteincentre.com.
⁴ Laboratory for Innovations in Microengineering (LiME), Department of Mechanical Engineering, University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada; Centre for Biomedical Research (CBR), University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada; Centre for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, 3800 Finnerty Rd., Victoria, BC, V8P 5C2, Canada. Electronic address: makbari@uvic.ca.

PMID: 28755975
DOI: 10.1016/j.tibtech.2017.06.006

Abstract

Interfacing mass spectrometry (MS) with microfluidic chips (μchip-MS) holds considerable potential to transform a clinician's toolbox, providing translatable methods for the early detection, diagnosis, monitoring, and treatment of noncommunicable diseases by streamlining and integrating laborious sample preparation workflows on high-throughput, user-friendly platforms. Overcoming the limitations of competitive immunoassays - currently the gold standard in clinical proteomics - μchip-MS can provide unprecedented access to complex proteomic assays having high sensitivity and specificity, but without the labor, costs, and complexities associated with conventional MS sample processing. This review surveys recent μchip-MS systems for clinical applications and examines their emerging role in streamlining the development and translation of MS-based proteomic assays by alleviating many of the challenges that currently inhibit widespread clinical adoption.

Keywords: clinical translation; lab-on-a-chip; mass spectrometry; microfluidics; proteomics.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Lab-On-A-Chip Devices*
Mass Spectrometry* / instrumentation
Mass Spectrometry* / methods
Microfluidic Analytical Techniques* / instrumentation
Microfluidic Analytical Techniques* / methods
Proteomics* / instrumentation
Proteomics* / methods