Gender Identification in Date Palm Using Molecular Markers

Faisal Saeed Awan; Maryam; Muhammad J Jaskani; Bushra Sadia

doi:10.1007/978-1-4939-7159-6_18

Gender Identification in Date Palm Using Molecular Markers

Methods Mol Biol. 2017:1638:209-225. doi: 10.1007/978-1-4939-7159-6_18.

Authors

Faisal Saeed Awan¹, Maryam², Muhammad J Jaskani², Bushra Sadia³

Affiliations

¹ Center of Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad, 3804, Pakistan. faisal.saeed@uaf.edu.pk.
² Institute of Horticultural Sciences, University of Agriculture, Faisalabad, 38040, Pakistan.
³ U.S.-Pakistan Center for Advanced Studies in Agriculture and Food Security (USPCAS-AFS), University of Agriculture, Faisalabad, Pakistan.

PMID: 28755226
DOI: 10.1007/978-1-4939-7159-6_18

Abstract

Breeding of date palm is complicated because of its long life cycle and heterozygous nature. Sexual propagation of date palm does not produce true-to-type plants. Sex of date palms cannot be identified until the first flowering stage. Molecular markers such as random amplified polymorphic DNA (RAPD), sequence-characterized amplified regions (SCAR), and simple sequence repeats (SSR) have successfully been used to identify the sex-linked loci in the plant genome and to isolate the corresponding genes. This chapter highlights the use of three molecular markers including RAPD, SCAR, and SSR to identify the gender of date palm seedlings.

Keywords: DNA markers; RAPD; SCAR; SSR; Sex identification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breeding / methods
DNA, Plant / genetics
Flowers / genetics
Genetic Markers / genetics*
Genome, Plant / genetics
Microsatellite Repeats / genetics
Phoeniceae / genetics*
Random Amplified Polymorphic DNA Technique / methods
Sex Determination Processes / genetics*

Substances

DNA, Plant
Genetic Markers