PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

B Molina-Moya; A Lacoma; N García-Sierra; S Blanco; L Haba; S Samper; J Ruiz-Manzano; C Prat; C Arnold; J Domínguez

doi:10.1038/s41598-017-06760-5

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping

Sci Rep. 2017 Jul 28;7(1):6777. doi: 10.1038/s41598-017-06760-5.

Authors

B Molina-Moya^{1

2}, A Lacoma^{1

2}, N García-Sierra¹, S Blanco^{1

2}, L Haba¹, S Samper^{2

3

4}, J Ruiz-Manzano^{5

2}, C Prat^{1

2}, C Arnold⁶, J Domínguez^{7

8}

Affiliations

¹ Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Carretera del Canyet s/n, 08916, Badalona, Spain.
² CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, 28029, Spain.
³ Instituto Aragonés de Ciencias de la Salud, Zaragoza, 50009, Spain.
⁴ Fundación Instituto de Investigación Sanitaria de Aragón, Zaragoza, 50009, Spain.
⁵ Servei de Pneumologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Carretera del Canyet s/n, 08916, Badalona, Spain.
⁶ Genomic Services and Development Unit, Public Health England, 61 Colindale Avenue, London, United Kingdom.
⁷ Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Institut d'Investigació Germans Trias i Pujol, Universitat Autònoma de Barcelona, Carretera del Canyet s/n, 08916, Badalona, Spain. jadominguez@igtp.cat.
⁸ CIBER Enfermedades Respiratorias (CIBERES), Instituto de Salud Carlos III, Madrid, 28029, Spain. jadominguez@igtp.cat.

Abstract

We developed a novel method, PyroTyping, for discrimination of Mycobacterium tuberculosis isolates combining pyrosequencing and IS6110 polymorphism. A total of 100 isolates were analysed with IS6110-restriction fragment length polymorphism (RFLP), spoligotyping, mycobacterial interspersed repetitive units - variable number tandem repeats (MIRU-VNTR), and PyroTyping. PyroTyping results regarding clustering or discrimination of the isolates were highly concordant with the other typing methods performed. PyroTyping is more rapid than RFLP and presents the same discriminatory power, thus, it may be useful for taking timely decisions for tuberculosis control.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Genotype
High-Throughput Nucleotide Sequencing / methods*
Minisatellite Repeats / genetics
Mycobacterium tuberculosis / classification
Mycobacterium tuberculosis / genetics*
Polymorphism, Restriction Fragment Length / genetics