Evaluation of OMNIgene®•SPUTUM for tuberculosis sample processing and liquid culture at a hospital laboratory in India

J Microbiol Methods. 2017 Oct:141:60-62. doi: 10.1016/j.mimet.2017.07.013. Epub 2017 Jul 25.

Abstract

This preliminary evaluation examined the reagent OMNIgene®•SPUTUM (OM-S) as a tool to eliminate NaOH/NALC processing prior to Middlebrook liquid culture for Mycobacterium tuberculosis (MTb). Twenty-seven manually split samples (OM-S-treated vs. NaOH/NALC) showed 100% agreement: 81.5% MTb-positive and 18.5% MTb-negative. On average, OM-S-treated specimens required 1.2 additional days to culture positivity.

Keywords: Laboratory reagent; Middlebrook liquid culture; Mycobacterium tuberculosis; OMNIgene®•SPUTUM; TB diagnostics.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteriological Techniques*
  • Culture Media
  • Humans
  • India / epidemiology
  • Indicators and Reagents
  • Laboratories, Hospital
  • Mycobacterium tuberculosis / growth & development
  • Mycobacterium tuberculosis / isolation & purification*
  • Sensitivity and Specificity
  • Specimen Handling / methods*
  • Sputum / microbiology*
  • Tuberculosis, Pulmonary / diagnosis*
  • Tuberculosis, Pulmonary / epidemiology
  • Tuberculosis, Pulmonary / microbiology

Substances

  • Culture Media
  • Indicators and Reagents