The UbiX-UbiD system: The biosynthesis and use of prenylated flavin (prFMN)

Arch Biochem Biophys. 2017 Oct 15:632:209-221. doi: 10.1016/j.abb.2017.07.014. Epub 2017 Jul 25.

Abstract

The UbiX-UbiD system consists of the flavin prenyltransferase UbiX that produces prenylated FMN that serves as the cofactor for the (de)carboxylase UbiD. Recent developments have provided structural insights into the mechanism of both enzymes, detailing unusual chemistry in each case. The proposed reversible 1,3-dipolar cycloaddition between the cofactor and substrate serves as a model to explain many of the key UbiD family features. However, considerable variation exists in the many branches of the UbiD family tree.

Keywords: (de)Carboxylase; Cycloaddition; Prenyl transferase; Prenylated FMN; UbiD; UbiX.

Publication types

  • Review

MeSH terms

  • Carboxy-Lyases* / chemistry
  • Carboxy-Lyases* / genetics
  • Carboxy-Lyases* / metabolism
  • Dimethylallyltranstransferase* / chemistry
  • Dimethylallyltranstransferase* / genetics
  • Dimethylallyltranstransferase* / metabolism
  • Escherichia coli Proteins* / chemistry
  • Escherichia coli Proteins* / genetics
  • Escherichia coli Proteins* / metabolism
  • Escherichia coli* / chemistry
  • Escherichia coli* / genetics
  • Escherichia coli* / metabolism
  • Flavins* / biosynthesis
  • Flavins* / chemistry
  • Flavins* / genetics
  • Flavoproteins* / chemistry
  • Flavoproteins* / genetics
  • Flavoproteins* / metabolism
  • Prenylation / physiology*

Substances

  • Escherichia coli Proteins
  • Flavins
  • Flavoproteins
  • Dimethylallyltranstransferase
  • 3-octaprenyl-4-hydroxybenzoate carboxy-lyase
  • Carboxy-Lyases
  • ubiX protein, E coli