Periodic Acid-Schiff Staining with Diastase

Dongtao A Fu; Martha Campbell-Thompson

doi:10.1007/978-1-4939-7163-3_14

Periodic Acid-Schiff Staining with Diastase

Methods Mol Biol. 2017:1639:145-149. doi: 10.1007/978-1-4939-7163-3_14.

Authors

Dongtao A Fu¹, Martha Campbell-Thompson²

Affiliations

¹ Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA.
² Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, Gainesville, FL, 32610, USA. mct@ufl.edu.

PMID: 28752454
DOI: 10.1007/978-1-4939-7163-3_14

Abstract

Periodic Acid-Schiff (PAS) with diastase (PAS-D) refers to the use of the PAS stain in combination with diastase, which is an enzyme that digests the glycogen. The purpose of using the PAS-D procedure is to differentiate glycogen from other PAS-positive elements in tissue samples. The PAS-D method is also used for periportal liver staining of AAT polymer inclusions that are seen in alpha-1 antitrypsin deficiency disease. Here, we describe the procedure of PAS-D staining in formalin-fixed, paraffin-embedded human liver tissues.

Keywords: Alpha-1 antitrypsin deficiency; Human liver; Periodic Acid-Schiff; Periodic Acid-Schiff reaction-diastase; Special stain.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Amylases / metabolism*
Humans
Liver / cytology
Liver / metabolism
Paraffin Embedding
Periodic Acid / metabolism*
Staining and Labeling / methods*

Substances

Periodic Acid
Amylases

Grants and funding

P01 DK058327/DK/NIDDK NIH HHS/United States