Immunohistochemistry Staining for Human Alpha-1 Antitrypsin

Dongtao A Fu; Martha Campbell-Thompson

doi:10.1007/978-1-4939-7163-3_13

Immunohistochemistry Staining for Human Alpha-1 Antitrypsin

Methods Mol Biol. 2017:1639:139-143. doi: 10.1007/978-1-4939-7163-3_13.

Authors

Dongtao A Fu¹, Martha Campbell-Thompson²

Affiliations

¹ Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, 1395 Center Drive, Gainesville, FL, 32610, USA.
² Department of Pathology, Immunology, and Laboratory Medicine, College of Medicine, University of Florida, 1395 Center Drive, Gainesville, FL, 32610, USA. mct@ufl.edu.

PMID: 28752453
DOI: 10.1007/978-1-4939-7163-3_13

Abstract

Immunohistochemistry (IHC) is a powerful immunology-based method that is used to study the location of proteins in cells and tissues. There have been numerous advancements in IHC technology that continually increase the sensitivity and specificity through which this method can be used to generate new discoveries. Similarly, Alpha-1 Antitrypsin (AAT) IHC can be used to study AAT protein expression within the human liver or exogenous AAT that is delivered through gene therapy. Here, we describe a highly sensitive method to detect the AAT antigen in formalin-fixed paraffin-embedded human or mouse tissues.

Keywords: Alpha-1 antitrypsin; Alpha-1 antitrypsin deficiency; Immunohistochemistry; Immunoperoxidase; Protein localization.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Formaldehyde
Humans
Immunohistochemistry / methods*
Paraffin Embedding
Staining and Labeling*
alpha 1-Antitrypsin / metabolism*

Substances

alpha 1-Antitrypsin
Formaldehyde

Grants and funding

P01 DK058327/DK/NIDDK NIH HHS/United States