Analysis of an RNA-seq Strand-Specific Library Sample Reveals a Complete Genome of Hardenbergia mosaic virus from Native Wisteria, an Indigenous Virus from Southwest Australia

Solomon Maina; Roger A C Jones

doi:10.1128/genomeA.00599-17

Analysis of an RNA-seq Strand-Specific Library Sample Reveals a Complete Genome of Hardenbergia mosaic virus from Native Wisteria, an Indigenous Virus from Southwest Australia

Genome Announc. 2017 Jul 27;5(30):e00599-17. doi: 10.1128/genomeA.00599-17.

Authors

Solomon Maina^{1

2}, Roger A C Jones^{3

4}

Affiliations

¹ School of Agriculture and Environment, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia.
² Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia.
³ Institute of Agriculture, Faculty of Science, University of Western Australia, Crawley, Western Australia, Australia roger.jones@uwa.edu.au.
⁴ Department of Agriculture and Food Western Australia, South Perth, Western Australia, Australia.

Abstract

Analysis of an RNA-seq strand-specific library revealed a complete genome of Hardenbergia mosaic virus (HarMV) from RNA extracted from a native wisteria (Hardenbergia comptoniana) plant from southwest Australia. We compared it with eight other complete HarMV genomes. It most resembled (85.8% nucleotide identity) the genome of HarMV isolate MD4-D.