A case of EDTA-related in vitro platelet degranulation

Ann Biol Clin (Paris). 2017 Aug 1;75(4):450-454. doi: 10.1684/abc.2017.1254.

Abstract

A 71 year-old woman is admitted to Le Mans hospital center for management of a chronic skin lesion. She has no personal nor familial bleeding history and does not take any medication. In peripheral blood collected with EDTA (ethylene diamine tetra-acetate), the platelet count is elevated and the blood film shows uniformly grey platelets. In sodium citrate-collected blood, platelets show no abnormality. We describe an EDTA-related artifact that is not to be mistaken for grey platelet syndrome.

Keywords: EDTA; Romanowsky stain; alpha-granules; artifact; blood film; grey platelets; sodium citrate.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Anticoagulants / pharmacology*
  • Artifacts*
  • Blood Platelets / drug effects*
  • Blood Platelets / physiology*
  • Cell Degranulation / drug effects*
  • Diagnosis, Differential
  • Diagnostic Errors / prevention & control
  • Edetic Acid / pharmacology*
  • Female
  • Gray Platelet Syndrome / blood
  • Gray Platelet Syndrome / diagnosis
  • Humans

Substances

  • Anticoagulants
  • Edetic Acid