Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016

Jesper Larsen; Marianne Sunde; Md Zohorul Islam; Anne Margrete Urdahl; Aina Steihaug Barstad; Anders Rhod Larsen; Carl Andreas Grøntvedt; Øystein Angen

doi:10.2807/1560-7917.ES.2017.22.28.30573

Evaluation of a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (MRSA), Denmark and Norway, 2014 to 2016

Euro Surveill. 2017 Jul 13;22(28):30573. doi: 10.2807/1560-7917.ES.2017.22.28.30573.

Authors

Jesper Larsen^{1

2}, Marianne Sunde^{2

3}, Md Zohorul Islam¹, Anne Margrete Urdahl³, Aina Steihaug Barstad³, Anders Rhod Larsen¹, Carl Andreas Grøntvedt³, Øystein Angen^{1

3}

Affiliations

¹ Statens Serum Institut, Copenhagen S, Denmark.
² These authors contributed equally as first authors.
³ The Norwegian Veterinary Institute, Oslo, Norway.

PMID: 28749335
PMCID: PMC5545765
DOI: 10.2807/1560-7917.ES.2017.22.28.30573

Abstract

We evaluated a widely used culture-based method for detection of livestock-associated meticillin-resistant Staphylococcus aureus (LA-MRSA) in samples collected from pigs and the environment inside pig stables in Denmark and Norway. Selective enrichment in tryptic soy broth containing cefoxitin and aztreonam led to a high ratio of false-negative results (26%; 57/221). On this basis, we recommend reconsidering the use of selective enrichment for detection of LA-MRSA in animal and environmental samples.

Keywords: MRSA; detection; laboratory surveillance; livestock; meticillin-resistant Staphylococcus aureus; sensitivity.

Publication types

Evaluation Study

MeSH terms

Animals
Culture Techniques / methods*
Denmark
Livestock
Methicillin-Resistant Staphylococcus aureus / isolation & purification*
Norway
Sensitivity and Specificity
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / veterinary
Sus scrofa
Swine
Swine Diseases / microbiology*