Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Lukhanyo Mekuto; Seteno K O Ntwampe; John B N Mudumbi; Enoch A Akinpelu; Maxwell Mewa-Ngongang

doi:10.1016/j.dib.2017.06.049

Metagenomic data of free cyanide and thiocyanate degrading bacterial communities

Data Brief. 2017 Jul 4:13:738-741. doi: 10.1016/j.dib.2017.06.049. eCollection 2017 Aug.

Authors

Lukhanyo Mekuto¹, Seteno K O Ntwampe¹, John B N Mudumbi¹, Enoch A Akinpelu¹, Maxwell Mewa-Ngongang¹

Affiliation

¹ Bioresource Engineering Research Group (BioERG), Department of Biotechnology, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000 South Africa.

Abstract

The data presented in this article contains the bacterial community structure of the free cyanide (CN^-) and thiocyanate (SCN^-) degrading organisms that were isolated from electroplating wastewater and synthetic SCN^- containing wastewater. PCR amplification of the 16S rRNA V1-V3 regions was undertaken using the 27F and 518R oligonucleotide primers following the metacommunity DNA extraction procedure. The PCR amplicons were processed using the illumina® reaction kits as per manufacturer׳s instruction and sequenced using the illumina® MiSeq-2000, using the MiSeq V3 kit. The data was processed using bioinformatics tools such as QIIME and the raw sequence files are available via NCBI׳s Sequence Read Archive (SRA) database.

Keywords: 16S rRNA gene; Cyanide degrading organisms; Metagenomics; Thiocyanate degrading organisms.