Imaging chemistry inside living cells by stimulated Raman scattering microscopy

Methods. 2017 Sep 1:128:119-128. doi: 10.1016/j.ymeth.2017.07.020. Epub 2017 Jul 23.

Abstract

Stimulated Raman scattering (SRS) microscopy is a vibrational imaging platform developed to visualize chemical content of a biological sample based on molecular vibrational fingerprints. With high-speed, high-sensitivity, and three-dimensional sectioning capability, SRS microscopy has been used to study chemical distribution, molecular transport, and metabolic conversion in living cells in a label-free manner. Moreover, aided with bio-orthogonal small-volume Raman probes, SRS microscopy allows direct imaging of metabolic activities of small molecules in living cells.

Keywords: Chemical imaging; In vivo imaging; Raman tag; Stimulated Raman scattering microscopy; Vibrational spectroscopy.

Publication types

  • Review
  • Research Support, Non-U.S. Gov't
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / diagnostic imaging
  • Humans
  • Imaging, Three-Dimensional / methods
  • Nonlinear Optical Microscopy / methods*
  • Skin / cytology*
  • Skin / diagnostic imaging*
  • Spectrum Analysis, Raman / methods*