A growing trend within nanomedicine has been the fabrication of self-delivering supramolecular nanomedicines containing a high and fixed drug content ensuring eco-friendly conditions. This study reports on green synthesis of silica nanoparticles (Si-NPs) using Azadirachta indica leaves extract as an effective chelating agent. X-ray diffraction analysis and Fourier transform-infra-red spectroscopic examination were studied. Scanning electron microscopy analysis revealed that the average size of particles formed via plant extract as reducing agent without any surfactant is in the range of 100-170 nm while addition of cetyltrimethyl ammonium bromide were more uniform with 200 nm in size. Streptomycin as model drug was successfully loaded to green synthesised Si-NPs, sustain release of the drug from this conjugate unit were examined. Prolong release pattern of the adsorbed drug ensure that Si-NPs have great potential in nano-drug delivery keeping the environment preferably biocompatible, future cytotoxic studies in this connection is helpful in achieving safe mode for nano-drug delivery.