Hepcidin is a key molecule involved in iron homeostasis. We measured hepcidin levels in 50 healthy children from Chandigarh, Northern India for establishing normal ranges. Hepcidin ranges (19.96-36.6 ng/mL; 0-2 years) and (9.54-36.15 ng/mL; 2-6 years) with mean (SD) of 32.5 (4.84) ng/mL, and 31.13 (6.62) ng/mL respectively were noted in study participants. The mean (SD) and ranges for plasma hepcidin in boys and girls in the study was 31.01 (6.71) ng/mL (9.54-36.6 ng/mL) and 32.7 (4.14) ng/mL (19-36.2 ng/mL), respectively.