High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)

Qinggong Tang; Yi Liu; Vassiliy Tsytsarev; Jonathan Lin; Bohan Wang; Udayakumar Kanniyappan; Zhifang Li; Yu Chen

doi:10.1364/BOE.8.002124

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)

Biomed Opt Express. 2017 Mar 9;8(4):2124-2137. doi: 10.1364/BOE.8.002124. eCollection 2017 Apr 1.

Authors

Qinggong Tang^{1

2}, Yi Liu^{1

2}, Vassiliy Tsytsarev³, Jonathan Lin¹, Bohan Wang¹, Udayakumar Kanniyappan¹, Zhifang Li⁴, Yu Chen¹

Affiliations

¹ Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742 USA.
² Contributed equally.
³ Department of Anatomy and Neurobiology, University of Maryland School of Medicine, Baltimore, MD 21201 USA.
⁴ Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.

Abstract

Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging in vivo.

Keywords: (170.2520) Fluorescence microscopy; (170.3010) Image reconstruction techniques; (170.3880) Medical and biological imaging; (170.6960) Tomography.