Genome-Wide Analysis of DNA Methylation in Hematopoietic Cells: DNA Methylation Analysis by WGBS

Methods Mol Biol. 2017:1633:137-149. doi: 10.1007/978-1-4939-7142-8_9.

Abstract

DNA methylation is a major epigenetic modification that regulates gene expression, genome imprinting, and development and has a role in diseases including cancer. There are various methods for whole-genome methylation profiling that differ in cost and resolution. Recent advances in high-throughput sequencing technologies coupled with bisulfite treatment enable absolute DNA methylation quantification and genome-wide single-nucleotide resolution analysis. In this chapter, we provide detailed protocols for whole-genome bisulfite sequencing (WGBS), which captures the complete methylome. Using WGBS, we are able to generate a reference DNA methylome for normal or malignant hematopoietic cells.

Keywords: CpG islands; DNA methylation; High-throughput sequencing; Whole-genome bisulfite sequencing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • CpG Islands
  • DNA Methylation*
  • DNA, Neoplasm / genetics*
  • Epigenomics
  • Genome, Human*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Leukemia, Myeloid, Acute / genetics*
  • Software
  • Sulfites / chemistry
  • Whole Genome Sequencing / methods*

Substances

  • DNA, Neoplasm
  • Sulfites
  • hydrogen sulfite