Structural Biology outside the box-inside the cell

Jürgen M Plitzko; Benjamin Schuler; Philipp Selenko

doi:10.1016/j.sbi.2017.06.007

Structural Biology outside the box-inside the cell

Curr Opin Struct Biol. 2017 Oct:46:110-121. doi: 10.1016/j.sbi.2017.06.007. Epub 2017 Jul 21.

Authors

Jürgen M Plitzko¹, Benjamin Schuler², Philipp Selenko³

Affiliations

¹ Max Planck Institute of Biochemistry, Department of Molecular Structural Biology, Am Klopferspitz 18, D-82152 Martinsried, Germany.
² Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
³ Leibniz Institute of Molecular Pharmacology (FMP Berlin), In-cell NMR Laboratory, Robert-Roessle Strasse 10, D-13125 Berlin, Germany. Electronic address: selenko@fmp-berlin.de.

PMID: 28735108
DOI: 10.1016/j.sbi.2017.06.007

Abstract

Recent developments in cellular cryo-electron tomography, in-cell single-molecule Förster resonance energy transfer-spectroscopy, nuclear magnetic resonance-spectroscopy and electron paramagnetic resonance-spectroscopy delivered unprecedented insights into the inner workings of cells. Here, we review complementary aspects of these methods and provide an outlook toward joint applications in the future.

Publication types

Review

MeSH terms

Cytological Techniques / methods*
Electron Spin Resonance Spectroscopy
Fluorescence Resonance Energy Transfer
Humans
Magnetic Resonance Spectroscopy