A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of →3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→ residues, partially sulfated at C-2 of →3)-α-l-Rhap-(1→ and C-3/C-4 of →2)-α-l-Rhap-(1→. The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked α-l-rhamnose and terminal β-d-glucuronic acid residues. The polysaccharide had strong antidiabetic activity assessed by glucose consumption, total cholesterol and triglyceride levels using human hepatocellular carcinoma (HepG2) and insulin-resistant HepG2 cells. The polysaccharide exhibited high anticoagulant property by activated partial thromboplastin time and thrombin time assays, and possessed high fibrin(ogen)olytic activity evaluated by plasminogen activator inhibitior-1, fibrin(ogen) degradation products and D-dimer levels using rats plasma. The investigation demonstrated that the polysaccharide from Monostroma angicava Kjellm was a novel sulfated rhamnan and could be a potential antidiabetic and anticoagulant polysaccharide.
Keywords: Anticoagulant property; Antidiabetic activity; Dimethyl sulfoxide (PubChem CID: 679); Heparin (PubChem CID: 25244225); Insulin (PubChem CID: 118984380); Metformin (PubChem CID: 4091); Monostroma angicava Kjellm; Palmitic acid (PubChem CID: 985); Pullunan (PubChem CID: 92024139); Pyridine (PubChem CID: 1049); Rhamnose (PubChem CID: 25310); Structure; Sulfated polysaccharide; Urokinase (PubChem CID: 54613018); d-Glucuronic acid (PubChem CID: 94715).
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