Blood coagulation evaluation of N-alkylated chitosan

Abstract

N-Alkylated chitosan (NACS) may improve the haemostatic efficiency of chitosan (CS). To study its coagulation capability and function, a series of NACS with various carbon chain lengths and substitution degrees (SD) of alkyl groups were synthesized and characterized by FTIR, NMR, and elemental analysis. Haemolysis and toxicity assays revealed that NACS showed good biocompatibility. In vitro blood clotting tests indicated that NACS had better haemostatic activity than CS, of which N-octadecyl CS with 3.85% SD showed the best results. Blood plasma coagulation tests showed that NACS was not favourable for activating coagulation factors. Platelet adhesion, intracellular Ca²⁺, and CD62p measurements demonstrated that the coagulation properties of NACS were not related to platelet activation. Erythrocyte adhesion examination indicated that blood coagulation of NACS may be attributable to its effects on erythrocytes. This study suggests that NACS is an ideal candidate for clotting.

Keywords: Acetic acid (PubChemCID: 176); Alkylated chitosan; Blood coagulation; CaCl2 (PubChemCID: 5284359); Deuterium chloride (PubChemCID: 522634); Deuterium oxide (PubChemCID: 24602); Dodecyl aldehyde (PubChemCID: 8194); Erythrocyte; Fluo-4 (PubChemCID: 25058176); Hexaldehyde (PubChemCID: 6184); Octadecane (PubChemCID: 11635); Platelet; Propidium iodide (PubChemCID: 104981); Sodium citrate (PubChemCID: 6224); Substitution degrees.