Performance of the β LACTA™ test for rapid detection of expanded-spectrum cephalosporin-non-susceptible Enterobacteriaceae

Georgia Vrioni; Vasiliki Mamali; Olympia Zarkotou; Evangelia Voulgari; Evangelia Dimitroulia; Katerina Themeli-Digalaki; Spyros Pournaras; Athanassios Tsakris

doi:10.1016/j.jgar.2017.05.018

Performance of the β LACTA™ test for rapid detection of expanded-spectrum cephalosporin-non-susceptible Enterobacteriaceae

J Glob Antimicrob Resist. 2017 Sep:10:285-288. doi: 10.1016/j.jgar.2017.05.018. Epub 2017 Jul 18.

Authors

Georgia Vrioni¹, Vasiliki Mamali², Olympia Zarkotou³, Evangelia Voulgari¹, Evangelia Dimitroulia⁴, Katerina Themeli-Digalaki², Spyros Pournaras⁵, Athanassios Tsakris¹

Affiliations

¹ Department of Microbiology, Medical School, National & Kapodistrian University of Athens, 75 M. Asias, 11527 Athens, Greece.
² Department of Microbiology, Tzaneio General Hospital, Afentouli & Zanni av., 18536 Piraeus, Greece.
³ Department of Biopathology, Evgenideion University Hospital, National & Kapodistrian University of Athens, 20 Papadiamantopoulou str., 11528 Athens, Greece.
⁴ Department of Microbiology, Medical School, National & Kapodistrian University of Athens, 75 M. Asias, 11527 Athens, Greece; Department of Biopathology, Evgenideion University Hospital, National & Kapodistrian University of Athens, 20 Papadiamantopoulou str., 11528 Athens, Greece.
⁵ Department of Microbiology, Medical School, National & Kapodistrian University of Athens, 75 M. Asias, 11527 Athens, Greece. Electronic address: spournaras@med.uoa.gr.

PMID: 28732790
DOI: 10.1016/j.jgar.2017.05.018

Abstract

Objectives: The aim of this study was to examine the performance of the chromogenic β LACTA™ test for the rapid detection of expanded-spectrum cephalosporin (ESC) non-susceptibility among Enterobacteriaceae in a region endemic for potent β-lactamases.

Methods: The β LACTA™ test was applied prospectively on 235 consecutive Enterobacteriaceae clinical isolates and 163 previously characterised ESC-non-susceptible Enterobacteriaceae producing a range of β-lactamases.

Results: The β LACTA™ test exhibited excellent sensitivity (96.1%) and specificity (98.5%) for the detection of ESC non-susceptibility in the 235 clinical isolates, which harboured mainly extended-spectrum β-lactamase (ESBL) and KPC- and NDM-type enzymes. Among the 163 challenged archived isolates, some false-negative or uninterpretable results were detected, mostly among ESC-non-susceptible isolates with AmpC/VIM/OXA-48-like enzymes.

Conclusion: β LACTA™ may be effectively applied in regions where ESC non-susceptibility among Enterobacteriaceae is mainly due to ESBL, KPC or NDM β-lactamases.

Keywords: Carbapenemase; Chromogenic cephalosporin; Detection; ESBL; β-Lactamase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / genetics*
Cephalosporin Resistance*
Enterobacteriaceae / drug effects
Enterobacteriaceae / genetics
Enterobacteriaceae / isolation & purification*
Enterobacteriaceae Infections / diagnosis*
Enterobacteriaceae Infections / microbiology
Humans
Microbial Sensitivity Tests
Prospective Studies
Reagent Kits, Diagnostic
Sensitivity and Specificity
Tertiary Healthcare

Substances

Bacterial Proteins
Reagent Kits, Diagnostic