Synthesis of PLGA-Lipid Hybrid Nanoparticles for siRNA Delivery Using the Emulsion Method PLGA-PEG-Lipid Nanoparticles for siRNA Delivery

Methods Mol Biol. 2017:1632:231-240. doi: 10.1007/978-1-4939-7138-1_15.

Abstract

The effective delivery of small interfering RNA (siRNA) to tumor cells remains a challenge for applications in cancer therapy. The development of polymeric nanoparticles with high siRNA loading efficacy has shown great potential for cancer targets. Double emulsion solvent evaporation technique is a useful tool for encapsulation of hydrophilic molecules (e.g., siRNA). Here we describe a versatile platform for siRNA delivery based on PLGA-PEG-cationic lipid nanoparticles by using the double emulsion method. The resulting nanoparticles show high encapsulation efficiency for siRNA (up to 90%) and demonstrate effective downregulation of the target genes in vitro and vivo.

Keywords: Cationic lipid; Double emulsion solvent evaporation; PLGA-PEG polymer; Polymeric nanoparticles; siRNA delivery.

MeSH terms

  • Emulsions*
  • Gene Transfer Techniques*
  • Lipids* / chemistry
  • Nanoparticles* / chemistry
  • Polyesters* / chemistry
  • Polyethylene Glycols* / chemistry
  • Polymers
  • RNA, Small Interfering / administration & dosage
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics*
  • Solvents / chemistry

Substances

  • Emulsions
  • Lipids
  • Polyesters
  • Polymers
  • RNA, Small Interfering
  • Solvents
  • polyethylene glycol-poly(lactide-co-glycolide)
  • Polyethylene Glycols